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Genomic instability

PARP1 and HPF1 team up to flag down DNA-repair machinery

Nature Structural & Molecular Biology volume 30, pages 568–569 (2023)Cite this article

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The enzymatic activity of PARP1—which adds chains of (poly-ADP)-ribose (PAR) to proteins—initiates DNA repair by leading to more-accessible chromatin and recruitment of PAR-dependent DNA-repair proteins. New work shows that these PARP1-catalysed functions are redirected by the auxiliary factor HPF1 in cells.

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Fig. 1: HPF1 modulates PARylation at sites of DNA damage.
Fig. 2: HPF1-mediated PARylation of histones leads to chromatin expansion and increased recruitment of DNA-repair factors.

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Acknowledgements

Funding was provided by the National Cancer Institute (grant R01 CA218255 to K.L.) and by the Howard Hughes Medical Institute (to K.L.).

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  1. University of Colorado Boulder, Boulder, CO, USA

    Johannes Rudolph & Karolin Luger

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  1. Johannes Rudolph
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  2. Karolin Luger
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Correspondence to Johannes Rudolph or Karolin Luger.

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Rudolph, J., Luger, K. PARP1 and HPF1 team up to flag down DNA-repair machinery. Nat Struct Mol Biol 30, 568–569 (2023). https://doi.org/10.1038/s41594-023-00987-9

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