Single-molecule live-cell imaging of the transcription dynamics in budding yeast cells revealed that the remodeling of different nucleosomes in the promoter of a gene regulates various kinetic steps of transcription. The measurements also showed that the TATA-binding protein competes with promoter-associated nucleosomes around the TATA element to activate transcription.
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References
Symmons, O. & Raj, A. What’s luck got to do with it: single cells, multiple fates, and biological nondeterminism. Mol. Cell 62, 788–802 (2016). A review describing several consequences of stochastic gene expression heterogeneity.
Brouwer, I. & Lenstra, T. L. Visualizing transcription: key to understanding gene expression dynamics. Curr. Opin. Chem. Biol. 51, 122–129 (2019). A review article that presents the results of imaging-based approaches to studying transcriptional bursting.
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This is a summary of: Brouwer, I., Kerklingh, E., van Leeuwen, F. & Lenstra, T. L. Dynamic epistasis analysis reveals how chromatin remodeling regulates transcriptional bursting. Nat. Struct. Mol. Biol. https://doi.org/10.1038/s41594-023-00981-1 (2023).
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Quantifying how promoter-associated nucleosomes regulate transcriptional bursting. Nat Struct Mol Biol 30, 577–578 (2023). https://doi.org/10.1038/s41594-023-00982-0
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DOI: https://doi.org/10.1038/s41594-023-00982-0
