Craspase is newly identified type III CRISPR–Cas system with two major components: the nuclease Cas7-11, and the protease TPR-CHAT. Craspases perform a delicate balancing act between nuclease and protease activity to achieve immune tolerance and defense in bacteria, and show promise as highly regulatable genome-editing tools.
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T.-M.F. was supported by the National Institutes of Health (NIH) National Institute of General Medical Sciences (grant 1R35GM147465) and A.D.R was supported by an NIH T32 (GM118291-05 and GM144293-01).
The authors declare no competing interests.
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Rish, A.D., Fu, TM. CRISPR–Cas has a new juggling act: interplay between nuclease and protease. Nat Struct Mol Biol 30, 126–128 (2023). https://doi.org/10.1038/s41594-022-00917-1