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Filling in the blanks: how the C-strand catches up to the G-strand at replicating telomeres using CST

CST is both an ssDNA-binding complex and a DNA Pol-α/primase cofactor that coordinates the switch from G-strand elongation to C-strand fill-in during telomere maintenance. Four papers in Nature Structural & Molecular Biology and Nature provide transformative insights into CST activity, providing a platform to understand lagging-strand synthesis genome wide.

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Fig. 1: Comparison of CST–Pol-α/primase structures from the de Lange, Lim and Feigon groups.
Fig. 2: Comparison of CST–Pol-α/primase structures from the Lim and Feigon groups, with emphasis on the ssDNA tracks.


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Research in the laboratory of D.S.W. is funded by the US National Institutes of Health (NIH; GM139274), and A.T.B. is supported by a fellowship provided by the NIH–University of Colorado Boulder (T32GM008759).

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Correspondence to Deborah S. Wuttke.

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Olson, C.L., Barbour, A.T. & Wuttke, D.S. Filling in the blanks: how the C-strand catches up to the G-strand at replicating telomeres using CST. Nat Struct Mol Biol 29, 730–733 (2022).

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