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TELOMERE REPLICATION

Filling in the blanks: how the C-strand catches up to the G-strand at replicating telomeres using CST

Nature Structural & Molecular Biology volume 29, pages 730–733 (2022)Cite this article

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CST is both an ssDNA-binding complex and a DNA Pol-α/primase cofactor that coordinates the switch from G-strand elongation to C-strand fill-in during telomere maintenance. Four papers in Nature Structural & Molecular Biology and Nature provide transformative insights into CST activity, providing a platform to understand lagging-strand synthesis genome wide.

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Fig. 1: Comparison of CST–Pol-α/primase structures from the de Lange, Lim and Feigon groups.
Fig. 2: Comparison of CST–Pol-α/primase structures from the Lim and Feigon groups, with emphasis on the ssDNA tracks.

References

  1. Zaug, A. J., Goodrich, K. J., Song, J. J., Sullivan, A. E. & Cech, T. C. Nature https://doi.org/10.1038/s41586-022-04930-8 (2022).

    Article  PubMed  Google Scholar 

  2. He, Q. et al. Nature https://doi.org/10.1038/s41586-022-05040-1 (2022).

  3. Cai, S. W. et al. Nat. Struct. Mol. Biol. https://doi.org/10.1038/s41594-022-00766-y (2022).

    Article  PubMed  Google Scholar 

  4. He, Y. et al. Nature https://doi.org/10.1038/s41586-022-04931-7 (2022).

    Article  PubMed  PubMed Central  Google Scholar 

  5. Linger, B. R. & Price, C. M. Crit. Rev. Biochem. Mol. Biol. 44, 434–446 (2010).

    Article  Google Scholar 

  6. Miyake, Y. et al. Mol. Cell 36, 193–206 (2009).

    Article  CAS  Google Scholar 

  7. Surovtseva, Y. V. et al. Mol. Cell 36, 207–218 (2009).

    Article  CAS  Google Scholar 

  8. Castell, D. E. et al. J. Biol. Chem. 284, 5807–5818 (2009).

    Article  Google Scholar 

  9. Chen, L. Y., Redon, S. & Lingner, J. Nature 488, 540–544 (2012).

    Article  CAS  Google Scholar 

  10. Chastain, M. et al. Cell Rep. 16, 1300–1314 (2016).

    Article  CAS  Google Scholar 

  11. Wang, F. et al. Cell Rep. 2, 1096–1103 (2012).

    Article  CAS  Google Scholar 

  12. Lim, C. J. & Cech, T. R. Nat. Rev. Mol. Cell Biol. 22, 283–298 (2021).

    Article  CAS  Google Scholar 

  13. Mirman, Z. et al. Nature 560, 112–116 (2018).

    Article  CAS  Google Scholar 

  14. Lim, C. J. et al. Science 368, 1081–1085 (2020).

    Article  CAS  Google Scholar 

  15. Hom, R. A. & Wuttke, D. S. Biochemistry 56, 4210–4218 (2017).

    Article  CAS  Google Scholar 

  16. Baranovskiy, A. G. et al. J. Biol. Chem. 291, 10006–10020 (2016).

    Article  CAS  Google Scholar 

  17. Zaug, A. J. et al. Nucleic Acids Res. 49, 11653–11665 (2021).

    Article  CAS  Google Scholar 

  18. He, Y. et al. Nature 593, 454–459 (2021).

    Article  CAS  Google Scholar 

  19. Zhong, F. L. et al. Cell 150, 481–494 (2012).

    Article  CAS  Google Scholar 

  20. Nandakumar, J. et al. Nature 492, 285–289 (2012).

    Article  CAS  Google Scholar 

  21. Sexton, A. N., Youmans, T. D. & Collins, K. J. Biol. Chem. 287, 34455–34464 (2012).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

Research in the laboratory of D.S.W. is funded by the US National Institutes of Health (NIH; GM139274), and A.T.B. is supported by a fellowship provided by the NIH–University of Colorado Boulder (T32GM008759).

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  1. Department of Biochemistry, University of Colorado Boulder, Boulder, CO, USA

    Conner L. Olson, Alexandra T. Barbour & Deborah S. Wuttke

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  1. Conner L. Olson
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  2. Alexandra T. Barbour
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  3. Deborah S. Wuttke
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Correspondence to Deborah S. Wuttke.

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Olson, C.L., Barbour, A.T. & Wuttke, D.S. Filling in the blanks: how the C-strand catches up to the G-strand at replicating telomeres using CST. Nat Struct Mol Biol 29, 730–733 (2022). https://doi.org/10.1038/s41594-022-00818-3

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