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MEMBRANE PROTEINS

The band 3–ankyrin multiprotein complex comes in from the cold

Two new papers describe the successful purification of the partially intact human native red blood cell band 3 multiprotein membrane complexes, providing information that the authors then use to capture the structures and interactions of multiple erythrocyte proteins using high-resolution cryo-EM.

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Fig. 1: A cryo-EM map of the domain organizations of band 3 dimer, protein 4.2 and ankyrin.
Fig. 2: Cryo-EM density map of a B3 ankyrin complexes.

References

  1. Vallese, F. et al. Nat. Struct. Mol. Biol. https://doi.org/10.1038/s41594-022-00792-w (2022).

    Article  Google Scholar 

  2. Xia, X., Liu, S. & Hong Zhou, Z. Nat. Struct. Mol. Biol. https://doi.org/10.1038/s41594-022-00779-7 (2022).

    Article  PubMed  Google Scholar 

  3. Bruce, L. J. et al. Blood 101, 4180–4188 (2003).

    CAS  Article  Google Scholar 

  4. van den Akker, E., Satchwell, T. J., Williamson, R. C. & Toye, A. M. Blood Cells Molec. Dis. 45, 1–8 (2010).

    Article  Google Scholar 

  5. Salomao, M. et al. Proc. Natl Acad. Sci. USA 105, 8026–8031 (2008).

    CAS  Article  Google Scholar 

  6. Perrotta, S., Gallagher, P. G. & Mohandas, N. Lancet 372, 1411–1426 (2008).

    CAS  Article  Google Scholar 

  7. Schofield, A. E., Reardon, D. M. & Tanner, M. J. A. Nature 355, 836–838 (1992).

    CAS  Article  Google Scholar 

  8. Bruce, L. J. Blood Cells Molec. Dis. 42, 216–222 (2009).

    CAS  Article  Google Scholar 

  9. Zhang, D., Kiyatkin, A., Bolin, J. T. & Low, P. S. Blood 96, 2925–2933 (2000).

    CAS  Article  Google Scholar 

  10. Arakawa, T. et al. Science 350, 680–684 (2015).

    CAS  Article  Google Scholar 

  11. Gruswitz, F. et al. Proc. Natl Acad. Sci. USA 107, 9638–9643 (2010).

    CAS  Article  Google Scholar 

  12. Toye, A. M. et al. Blood 105, 4088–4095 (2005).

    CAS  Article  Google Scholar 

  13. Mankelow, T. J., Satchwell, T. J. & Burton, N. M. Blood Cells Molec. Dis. 49, 1–10 (2012).

    CAS  Article  Google Scholar 

Download references

Acknowledgements

The author thanks H. Zhou and O. Clarke for their generation of Figs. 1 and 2, respectively, and T. Satchwell for critical reading of this article.

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Correspondence to Ashley M. Toye.

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Toye, A.M. The band 3–ankyrin multiprotein complex comes in from the cold. Nat Struct Mol Biol 29, 621–623 (2022). https://doi.org/10.1038/s41594-022-00789-5

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