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DNA REPLICATION AND REPAIR

The partner-swapping sliding clamp loader exposed

Eukaryotes possess several clamp loaders comprising four common subunits and a fifth subunit unique to each complex. The RFC-A–E clamp loader loads the PCNA clamp at 3′-recessed structures for DNA replication. However, swapping a single subunit, Rad24, for RFC-A yields a clamp loader that prefers the 911 clamp and 5′-recessed DNA. Three new studies reveal detailed views of the clamp loading reaction and provide insights into substrate preferences of each loader.

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Fig. 1: Sliding clamps and clamp loaders.
Fig. 2: The DNA replication and damage-response clamp loaders.

References

  1. Yao, N. Y. & O’Donnell, M. E. Subcell. Biochem. 96, 233–258 (2020).

    Article  Google Scholar 

  2. Bell, S. P. & Labib, K. Genetics 203, 1027–1067 (2016).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  3. Kelch, B. A. Biopolymers 105, 532–546 (2016).

    CAS  Article  PubMed  Google Scholar 

  4. Bowman, G. D., Goedken, E. R., Kazmirski, S. L., O’Donnell, M. & Kuriyan, J. FEBS Lett. 579, 863–867 (2005).

    CAS  Article  PubMed  Google Scholar 

  5. Lee, K. & Park, S. H. Exp. Mol. Med. 52, 1948–1958 (2020).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  6. Majka, J. & Burgers, P. M. J. Prog. Nucleic Acid Res. 78, 227–260 (2004).

    CAS  Article  Google Scholar 

  7. Choe, K. N. & Moldovan, G.-L. Mol. Cell 65, 380–392 (2017).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  8. Attali, I., Botchan, M. R. & Berger, J. M. S. Annu. Rev. Biochem. 90, 1–30 (2021).

    Article  Google Scholar 

  9. Zheng, F., Georgescu, R. E., Yao, N. Y., O’Donnell, M. E. & Li, H. Nat. Struct. Mol. Biol. https://doi.org/10.1038/s41594-022-00742-6 (2022).

    Article  PubMed  Google Scholar 

  10. Castaneda, J. C., Schrecker, M., Remus, D. & Hite, R. K. Nat. Struct. Mol. Biol. https://doi.org/10.1038/s41594-022-00741-7 (2022).

    Article  PubMed  Google Scholar 

  11. Gaubitz, C. et al. eLife 11, e74175 (2022).

    Article  PubMed  PubMed Central  Google Scholar 

  12. Parrilla-Castellar, E. R., Arlander, S. J. H. & Karnitz, L. DNA Rep. 3, 1009–1014 (2004).

    CAS  Article  Google Scholar 

  13. Khan, Y. A., White, K. I. & Brunger, A. T. Crit. Rev. Biochem. Mol. https://doi.org/10.1080/10409238.2021.1979460 (2021).

    Article  Google Scholar 

  14. Arbel, M., Choudhary, K., Tfilin, O. & Kupiec, M. Genes (Basel) 12, 1812 (2021).

    CAS  Article  Google Scholar 

  15. Ellison, V. & Stillman, B. PLoS Biol. 1, e33 (2003).

    Article  PubMed  PubMed Central  Google Scholar 

  16. Majka, J., Binz, S. K., Wold, M. S. & Burgers, P. M. J. J. Biol. Chem. 281, 27855–27861 (2006).

    CAS  Article  PubMed  Google Scholar 

  17. Zou, L., Liu, D. & Elledge, S. J. Proc. Natl Acad. Sci. USA 100, 13827–13832 (2003).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  18. Yoshida, K. & Fujita, M. Cell. Mol. Life Sci. 78, 6763–6773 (2021).

    CAS  Article  PubMed  Google Scholar 

  19. Lanz, M. C., Dibitetto, D. & Smolka, M. B. EMBO J. 38, e101801 (2019).

    Article  PubMed  PubMed Central  Google Scholar 

  20. Waterman, D. P., Haber, J. E. & Smolka, M. B. Annu. Rev. Biochem. 89, 103–133 (2020).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  21. Kelch, B. A., Makino, D. L., O’Donnell, M. & Kuriyan, J. Science 334, 1675–1680 (2011).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  22. Jeruzalmi, D., O’Donnell, M. & Kuriyan, J. Cell 106, 429–441 (2001).

    CAS  Article  PubMed  Google Scholar 

  23. Jeruzalmi, D. et al. Cell 106, 417–428 (2001).

    CAS  Article  PubMed  Google Scholar 

  24. Bowman, G. D., O’Donnell, M. & Kuriyan, J. Nature 429, 724–730 (2004).

    CAS  Article  PubMed  Google Scholar 

  25. Simonetta, K. R. et al. Cell 137, 659–671 (2009).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

Download references

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Jeruzalmi, D. The partner-swapping sliding clamp loader exposed. Nat Struct Mol Biol 29, 283–286 (2022). https://doi.org/10.1038/s41594-022-00761-3

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