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Tandem regulation of MutS activity by ATP and DNA during MMR initiation

An elegant cryo-EM and biophysical study unveils the conformational changes of the E. coli MutS mismatch repair factor. This provides a nice follow-up to the recent report from the same group characterizing how MutS scans DNA without initiating repair on correctly base-paired DNA and recruits MutL upon encountering a mismatch.

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Fig. 1: Borsellini et al. report four cryo-EM structures of MutS in its apo form (grey) with ADP (orange), ATP or ATP analog (red) nucleotides.


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J.B.C. thanks the French National Research Agency for support (ANR-20-CE11-0026, ANR-17-CE12-0020 and ANR- 18-CE44-0008).

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Correspondence to Jean Baptiste Charbonnier.

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Charbonnier, J.B. Tandem regulation of MutS activity by ATP and DNA during MMR initiation. Nat Struct Mol Biol 29, 5–7 (2022).

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