Extended Data Fig. 1: Remodeling activities of the crosslinked and reduced nucleosomes. | Nature Structural & Molecular Biology

Extended Data Fig. 1: Remodeling activities of the crosslinked and reduced nucleosomes.

From: Reply to: Histone dynamics play a critical role in SNF2h-mediated nucleosome sliding

Extended Data Fig. 1

(a) Non-reducing SDS-PAGE analysis of the crosslinked (X) and reduced forms of the H3L82C/H4V81C octamer. Treatment of the crosslinked nucleosome with 100 mM DTT at 37 °C for 1 h and at 55 °C for 2 h resulted in reversion of ~25% and ~50% of the samples into the reduced form. (b) Nucleosome centering activities of the wild type (WT), crosslinked (X) and crosslinked and then reduced by DTT at 37 °C for 1 h (X + DTT) samples. Error bars indicate s.d. of the means (n = 3 independent experiments). Representative gels are shown below. The sliding rate constants are indicated. (c) Nucleosome centering activities of the WT, crosslinked (X) and crosslinked and then reduced by DTT at 55 °C for 2 h (X + DTT) samples.

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