Extended Data Fig. 1: Preparation of crosslinked and reduced histone octamers. | Nature Structural & Molecular Biology

Extended Data Fig. 1: Preparation of crosslinked and reduced histone octamers.

From: Histone dynamics play a critical role in SNF2h-mediated nucleosome sliding

Extended Data Fig. 1

SDS-PAGE gels of Xenopus laevis of histones used to prepare all nucleosomes in this study. (a) Wild type (WT) Histone H2A-H2B dimer as well as H3C110A histone octamer and H3C110A sCX2 (H3 L82C, H4 V81C) histone octamer oxidized using copper phenanthroline (CuPhe) or oxidized glutathione (GSSG). (b) The same samples used in (A) treated with 100 mM DTT in order to reduce the disulfide bond. Uncropped images are available as source data files. SDS-PAGE analysis was only performed once for all samples.

Source data

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