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Water route for proton pumping in mitochondrial complex I

A new cryo-EM structure of mitochondrial complex I reveals ordered water molecules that connect key elements of the proton-translocating machinery. Analysis of the ubiquinone-binding site of complex I offers insights into the mechanism of catalytic turnover and the regulation of this essential metabolic enzyme.

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Fig. 1: The Yarrowia lipolytica complex I structure determined by Grba and Hirst (PDB 6YJ4).


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Correspondence to Alexander Galkin.

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Galkin, A. Water route for proton pumping in mitochondrial complex I. Nat Struct Mol Biol 27, 1–3 (2020).

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