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CRISPR

The final cut: Cas9 editing

The ability of CRISPR-Cas9 to accurately and efficiently target and cleave any segment of double-stranded DNA based solely on the sequence of its loaded guide RNA has revolutionized genome editing. While many structural studies have shed light on the atomic details of DNA targeting, structures of the enzyme poised to perform catalysis have remained elusive. In this issue, Zhu, Clarke, Puppala et al. provide snapshots of the enzyme in action as it performs concerted cleavage of a target DNA1.

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Fig. 1: Snapshots of Cas9 in action.

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Acknowledgements

I thank M. O’Connell and S. Sternberg for critical reading of this article.

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Correspondence to David W. Taylor.

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D.W.T. is an inventor on a CRISPR-related patent.

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Taylor, D.W. The final cut: Cas9 editing. Nat Struct Mol Biol 26, 669–670 (2019). https://doi.org/10.1038/s41594-019-0267-1

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