MICROTUBULES

Cytoskeletal cryptography: structure and mechanism of an eraser

Article metrics

  • 518 Accesses

The ‘tubulin code’, a set of post-translational modifications to the microtubule cytoskeleton that include removal of the C-terminal Tyr of α-tubulin, regulates the biological function of the polymer. Three studies now report structures of VASH1–SVBP and VASH2–SVBP heterodimers and provide insights into how these proteases recognize and cleave the terminal Tyr of α-tubulin.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

Fig. 1: The proteases VASH1–SVBP and VASH2–SVBP erase the ultimate Tyr from α-tubulin.
Fig. 2: Comparative view of active sites of complexes of VASH1–SVBP and VASH2–SVBP plus inhibitor or α-tubulin.

References

  1. 1.

    Aillaud, C. et al. Science 358, 1448–1453 (2017).

  2. 2.

    Nieuwenhuis, J. et al. Science 358, 1453–1456 (2017).

  3. 3.

    Wang, N. et al. Nat. Struct. Mol. Biol. https://doi.org/10.1038/s41594-019-0241-y (2019).

  4. 4.

    Li, F. et al. Nat. Struct. Mol. Biol. https://doi.org/10.1038/s41594-019-0242-x (2019).

  5. 5.

    Adamopoulos, A. et al. Nat. Struct. Mol. Biol. https://doi.org/10.1038/s41594-019-0254-6 (2019).

  6. 6.

    Gadadhar, S., Bodakuntla, S., Natarajan, K. & Janke, C. J. Cell Sci. 130, 1347–1353 (2017).

  7. 7.

    Roll-Mecak, A. Curr. Opin. Cell Biol. 56, 102–108 (2019).

  8. 8.

    Hallak, M. E., Rodriguez, J. A., Barra, H. S. & Caputto, R. FEBS Lett. 73, 147–150 (1977).

  9. 9.

    Sanchez-Pulido, L. & Ponting, C. P. Bioinformatics 32, 1441–1445 (2016).

  10. 10.

    Chatterjee, D., Boyd, C. D., O’Toole, G. A. & Sondermann, H. J. Bacteriol. 194, 4415–4425 (2012).

  11. 11.

    Liao, S. Cell Res. https://doi.org/10.1038/s41422-019-0187-y (2019).

Download references

Author information

Correspondence to Kevin C. Slep.

Ethics declarations

Competing interests

The author declares no competing interests.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark