a, Size exclusion chromatography profile of V2c 4A mutant. The V2c 4A–SVBP complex was compared with the wild type V2c–SVBP complex using size exclusion chromatography. The profile suggests that the V2 4A mutant is indeed folded properly. b–h, Most of the complex mutants used in this study were also analyzed and compared with the wild type V2–SVBP complexes using size exclusion chromatography. The profiles also suggest that all V2 variants are folded properly.