Supplementary Figure 4: Analysis of the initial rates of activity of purified catalytic site mutants. | Nature Structural & Molecular Biology

Supplementary Figure 4: Analysis of the initial rates of activity of purified catalytic site mutants.

From: Structural basis of tubulin detyrosination by the vasohibin–SVBP enzyme complex

Supplementary Figure 4

a–j, In vitro detyrosination assays were performed with purified V2–SVBP (composed of wild type or mutated full length V2 and wild-type SVBP) at the concentrations indicated using [14C]-tyrosinated taxol-stabilized microtubules (2 μM). n = 2–4 data points per time point and V2–SVBP concentration. n.d., not determined. Linear regression lines are shown in black. All the source files are available in Supplementary Data Set 2. The correct folding of proteins was assessed by size exclusion chromatography (Supplementary Fig. 6b–h).

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