a–j, In vitro detyrosination assays were performed with purified V2–SVBP (composed of wild type or mutated full length V2 and wild-type SVBP) at the concentrations indicated using [14C]-tyrosinated taxol-stabilized microtubules (2 μM). n = 2–4 data points per time point and V2–SVBP concentration. n.d., not determined. Linear regression lines are shown in black. All the source files are available in Supplementary Data Set 2. The correct folding of proteins was assessed by size exclusion chromatography (Supplementary Fig. 6b–h).