Supplementary Figure 7: Interactions of the distal part of the capped RNA primer. | Nature Structural & Molecular Biology

Supplementary Figure 7: Interactions of the distal part of the capped RNA primer.

From: Structural snapshots of actively transcribing influenza polymerase

Supplementary Figure 7

Interactions of the distal part of the capped RNA primer (m7GpppGAAUGC…) with specific residues (yellow sticks) of the cap-binding (orange), PB2-midlink (violet) and PB2-N (ruby) domains as observed in the mixed initiation state structures. Nucleotides 2-AAU-4 are stacked on each other and sandwiched between PB2/Tyr434 of the cap-binding domain and a salt-bridge between PB2/Arg217 and Glu155 of the PB2 N2 domain. G5 is in a separate pocket stacking between of PB2/Arg146 and Arg425 and makes base contacts with PB1/Glu227.

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