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tRNA recycling on stalled ribosomes

In the ribosome-associated quality control (RQC) pathway, stalled ribosomes are ubiquitinated and dissociated into subunits. The nascent protein chain associated with the 60S ribosomal subunit is ubiquitinated by the E3 ligase Listerin (Ltn1) and is released from tRNA by ANKZF1 (Vms1) for proteasomal degradation. Shao and colleagues now report that ANKZF1 (Vms1)-cleaved tRNAs are recycled via a two-step process that requires the removal of a terminal 2′,3′-cyclic phosphate and the addition of CCA by TRNT1.

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Fig. 1: Mechanism for recycling tRNAs on stalled ribosomes.


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Correspondence to Toshifumi Inada.

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Inada, T. tRNA recycling on stalled ribosomes. Nat Struct Mol Biol 26, 340–342 (2019).

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