Supplementary Figure 5: Transcriptional interference at the Xist–Tsix locus. | Nature Structural & Molecular Biology

Supplementary Figure 5: Transcriptional interference at the XistTsix locus.

From: A symmetric toggle switch explains the onset of random X inactivation in different mammals

Supplementary Figure 5

(a–f) TXY and TXY∆A ESCs were treated with doxycycline for 24 hours and nascent transcription of Xist and Tsix (5′ and 3′) was assessed by RNA FISH. (ac) Quantification of 3 biological replicates, where each dot represents the measured signal intensities of a single allele. Grey lines indicate the detection threshold estimated from negative control regions. (df) Box plots of Tsix signal intensity at Xist+ (green) and Xist- alleles (black) in the two cell lines as indicated for the data shown in (a-c); dotted lines indicate the detection threshold (center line, median; box limits, upper and lower quartiles; whiskers, most extreme data points not considered outliers; points, outliers).

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