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CRISPR

Enzymatic anti-CRISPRs improve the bacteriophage arsenal

Bacteriophage-encoded anti-CRISPR (Acr) proteins were previously thought to inhibit CRISPR-mediated immunity by acting as physical barriers against the binding or cleavage of DNA. Two new studies report that recently discovered type V Acr proteins use enzymatic activities to shut down the Cas12a endonuclease, providing a multi-turnover ‘off switch’ for CRISPR-based immunity and technology.

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Correspondence to Dipali G. Sashital.

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Further reading

Fig. 1: Alternative mechanisms for disabling Cas effectors.
Fig. 2: AcrVA1 and AcrVA5 are enzymatic inhibitors of Cas12a.