Supplementary Figure 8: Neutralization of rVSV-EBOV GP and rVSV-EBOV GPΔβ17-β18 by ADI-15946 and affinity variants. | Nature Structural & Molecular Biology

Supplementary Figure 8: Neutralization of rVSV-EBOV GP and rVSV-EBOV GPΔβ17-β18 by ADI-15946 and affinity variants.

From: Structural basis of broad ebolavirus neutralization by a human survivor antibody

Supplementary Figure 8

(a) ADI-15946 shows increased neutralization of an rVSV-EBOV GP variant bearing the deletion of residues 187-198 (GPΔβ17-β18) compared to rVSV bearing wild-type GP (WT)—same as figure 3C. (b) Deletion of the β17-β18 loop does not have a major impact on neutralization by KZ52. (c-d) ADI-15946 affinity variants 46M1, 46M2, and 46M3 also show enhanced neutralization of rVSV-EBOV GPΔβ17-β18. (f) The IC50 values are tabulated from each of the experiments graphed in a-e. (a-e) Mean ± SD; n = 6 biologically independent samples in each figure panel.

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