Supplementary Figure 4: FVM09 competition biolayer interferometry assays. | Nature Structural & Molecular Biology

Supplementary Figure 4: FVM09 competition biolayer interferometry assays.

From: Structural basis of broad ebolavirus neutralization by a human survivor antibody

Supplementary Figure 4

EBOV GP was loaded onto Nickel-NTA biosensors followed by two association steps of test antibodies. Pre-binding of FVM09 does not interfere with subsequent association of ADI-15946 (a) or KZ52 (c). In a converse experiment, pre-binding of ADI-15946 (b) or KZ52 (d) to GP does not interfere with subsequent binding of FVM09. In the second association steps, the first binder is maintained at the same concentration as in the first association step with addition of the competing antibody. All measurements were performed with antibodies at 330 nM concentration.