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Biological Techniques

Mapping the diploid genome, one cell at a time

Attempts to develop a method for 3D genome reconstruction of single cells have been frustrated by the inability to distinguish between chromosome homologs. A novel Hi-C workflow uses haplotype imputation to map the nuclear organization of single diploid cells.

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Correspondence to Blake A. Caldwell or Marisa S. Bartolomei.

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Fig. 1: 3D genome technologies.