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Biological Techniques

Mapping the diploid genome, one cell at a time

Nature Structural & Molecular Biology volume 25, pages 994–995 (2018)Cite this article

Attempts to develop a method for 3D genome reconstruction of single cells have been frustrated by the inability to distinguish between chromosome homologs. A novel Hi-C workflow uses haplotype imputation to map the nuclear organization of single diploid cells.

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Fig. 1: 3D genome technologies.

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Authors and Affiliations

  1. Epigenetics Institute, Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA

    Blake A. Caldwell & Marisa S. Bartolomei

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  1. Blake A. Caldwell
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  2. Marisa S. Bartolomei
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Correspondence to Blake A. Caldwell or Marisa S. Bartolomei.

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The authors declare no competing interests.

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Caldwell, B.A., Bartolomei, M.S. Mapping the diploid genome, one cell at a time. Nat Struct Mol Biol 25, 994–995 (2018). https://doi.org/10.1038/s41594-018-0149-y

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