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Reply to ‘Concerns with yeast mitochondrial ADP/ATP carrier’s integrity in DPC’ and ‘Dynamics and interactions of AAC3 in DPC are not functionally relevant’

Nature Structural & Molecular Biology volume 25, pages 749–750 (2018)Cite this article

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Yang et al. reply: In their correspondence, Kurauskas et al.1 and King et al.2 claim that the protein we used to measure the micro- to millisecond dynamics of yeast ADP/ATP carrier (yAAC3) in our NSMB study3 was not in a functional, native state and thus the data have no biological relevance.

We strongly believe that yAAC3 in dodecylphosphocholine (DPC), although suboptimally folded and unable to generate a native dissociation constant (K d) for ligand CATR, is nevertheless in a state that can provide qualitative information that is relevant for functional investigations. A suboptimally folded state is in many ways expected for detergent-solubilized membrane proteins; the detergent could have loosened the structure, effectively making the ligand-binding site more dynamic. Even minor destabilization of the binding site can have a dramatic effect on K d. The key question is, can we learn anything meaningful from the yAAC3 sample used for NMR analyses? NMR allows direct K d measurements, but this does not mean that a sample must have physiological K d to be considered suitable for structural investigations.

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References

  1. Kurauskas, V. et al. Nat. Struct. Mol. Biol. https://doi.org/10.1038/s41594-018-0127-4 (2018).

  2. King, M. S., Crichton, P. G., Ruprecht, J. J. & Kunji, E. R. Nat. Struct. Mol. Biol. https://doi.org/10.1038/s41594-018-0125-6 (2018).

  3. Brüschweiler, S., Yang, Q., Run, C. & Chou, J. J. Nat. Struct. Mol. Biol. 22, 636–641 (2015).

    Article  Google Scholar 

  4. Berardi, M. J. & Chou, J. J. Cell Metab. 20, 541–552 (2014).

    Article  CAS  Google Scholar 

  5. Run, C., Yang, Q., Liu, Z., OuYang, B. & Chou, J. J. Structure 23, 1394–1403 (2015).

    Article  CAS  Google Scholar 

  6. Zhao, L. et al. Structure 25, 1371–1379 (2017).

    Article  CAS  Google Scholar 

  7. Babot, M., Blancard, C., Zeman, I., Lauquin, G. J. & Trézéguet, V. Biochemistry 51, 7348–7356 (2012).

    Article  CAS  Google Scholar 

  8. Korzhnev, D. M., Neudecker, P., Mittermaier, A., Orekhov, V. Y. & Kay, L. E. J. Am. Chem. Soc. 127, 15602–15611 (2005).

    Article  CAS  Google Scholar 

  9. Kovrigin, E. L., Kempf, J. G., Grey, M. J. & Loria, J. P. J. Magn. Reson. 180, 93–104 (2006).

    Article  CAS  Google Scholar 

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Author notes

  1. Qin Yang

    Present address: Aptitude Medical Systems, Inc., Santa Barbara, CA, USA

  2. Sven Brüschweiler

    Present address: Department for Structural and Computational Biology, Max F. Perutz Laboratories, University of Vienna, Vienna, Austria

  3. These authors contributed equally: Qin Yang, Sven Brüschweiler.

Authors and Affiliations

  1. Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA

    Qin Yang, Sven Brüschweiler, Linlin Zhao & James J. Chou

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  1. Qin Yang
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Correspondence to James J. Chou.

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Yang, Q., Brüschweiler, S., Zhao, L. et al. Reply to ‘Concerns with yeast mitochondrial ADP/ATP carrier’s integrity in DPC’ and ‘Dynamics and interactions of AAC3 in DPC are not functionally relevant’. Nat Struct Mol Biol 25, 749–750 (2018). https://doi.org/10.1038/s41594-018-0126-5

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