Supplementary Figure 3: HIV-1 virions induce a global positive Bcc on COS7 cells expressing CD4-mOrange—CXCR4-mTFP1. | Nature Structural & Molecular Biology

Supplementary Figure 3: HIV-1 virions induce a global positive Bcc on COS7 cells expressing CD4-mOrange—CXCR4-mTFP1.

From: A dynamic three-step mechanism drives the HIV-1 pre-fusion reaction

Supplementary Figure 3

a, An overall increase in brightness was seen in both channels (CD4 and CXCR4) all across the cell (second row) and in Bcc (last row) upon HXB2-HIV virion addition. Scale bar, 2 μm. b, Box plot diagram for the average oligomeric states for both CD4 (red dots) and CXCR4 (green dots) throughout the cell before and after addition of untreated HIV-1 (HXB2) virions or HIV-1 HXB2 virions treated with SQV (50 nM). c, Graph representing CD4 versus CXCR4 oligomeric states before (red dots) and after (green dots) HIV-1.

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