Supplementary Figure 2: HIV-1 addition on COS7 cells expressing CD4-mOrange—CXCR4-mTFP1 does not induce positive FRET. | Nature Structural & Molecular Biology

Supplementary Figure 2: HIV-1 addition on COS7 cells expressing CD4-mOrange—CXCR4-mTFP1 does not induce positive FRET.

From: A dynamic three-step mechanism drives the HIV-1 pre-fusion reaction

Supplementary Figure 2

Fluorescence lifetime imaging microscopy (FLIM) was measured on COS7 cells expressing CD4-mOrange and CXCR4-mTFP1 before and after addition of HIV-1HXB2-Gag-iCherry virions. The CXCR4-mTFP1 lifetime remained the same (~2.4 ns), indicating that no measurable FRET occurred (bottom).

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