Supplementary Figure 1: Cross number and brightness applied to detect CD4 and co-receptor interactions. | Nature Structural & Molecular Biology

Supplementary Figure 1: Cross number and brightness applied to detect CD4 and co-receptor interactions.

From: A dynamic three-step mechanism drives the HIV-1 pre-fusion reaction

Supplementary Figure 1

Cartoon depicting the fluorescence intensity fluctuation traces coming from two different channels (CD4 and co-receptors), that is, homotypic interactions. The amplitude of the fluorescence fluctuation is related to the oligomeric state of the proteins under study. The cross-variance analysis renders information about heterotypic interactions. If heterotypic interactions are detected, colocalization analysis between these regions and HIV-1 virions is performed. Subsequently, the oligomeric state for these regions is extracted, obtaining the time-resolved oligomeric state of the interaction upon addition of the HIV-1 virions on the surface of live cells.

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