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RNA decay

Molecular autopsy provides evidence for widespread ribosome-phased mRNA fragmentation

Nature Structural & Molecular Biology volume 25, pages 299–301 (2018)Cite this article

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Recent developments in transcriptome-wide sequencing technologies have enabled the identification of cellular mRNA decay intermediates. Although canonical mRNA decay has been shown to occur by deadenylation followed by decapping and subsequent exonucleolytic decay from both mRNA ends, a study by Mourelatos and colleagues now defines mRNA fragments that are generated on polysomes by endonucleolytic cleavages phased by the associated ribosome.

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Fig. 1: Model for HeLa cell ribothrypsis.

References

  1. Garneau, N. L., Wilusz, J. & Wilusz, C. J. Nat. Rev. Mol. Cell Biol. 8, 113–126 (2007).

    Article  CAS  PubMed  Google Scholar 

  2. Parker, R. Genetics 191, 671–702 (2012).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Roy, B. & Jacobson, A. Trends Genet. 29, 691–699 (2013).

    Article  CAS  PubMed  Google Scholar 

  4. Steitz, J. A. Nature 224, 957–964 (1969).

    Article  CAS  PubMed  Google Scholar 

  5. Kozak, M. & Shatkin, A. J. J. Biol. Chem. 251, 4259–4266 (1976).

    CAS  PubMed  Google Scholar 

  6. Schoenberg, D. R. & Maquat, L. E. Nat. Rev. Genet. 13, 246–259 (2012).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Shoemaker, C. J. & Green, R. Nat. Struct. Mol. Biol. 19, 594–601 (2012).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Hanson, G. & Coller, J. Nat. Rev. Mol. Cell Biol. 19, 20–30 (2018).

    Article  CAS  PubMed  Google Scholar 

  9. Sheth, U. & Parker, R. Science 300, 805–808 (2003).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Coller, J. & Parker, R. Cell 122, 875–886 (2005).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Hu, W., Sweet, T. J., Chamnongpol, S., Baker, K. E. & Coller, J. Nature 461, 225–229 (2009).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Pelechano, V., Wei, W. & Steinmetz, L. M. Cell 161, 1400–1412 (2015).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Hu, W., Petzold, C., Coller, J. & Baker, K. E. Nat. Struct. Mol. Biol. 17, 244–247 (2010).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Doma, M. K. & Parker, R. Nature 440, 561–564 (2006).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Simms, C. L., Yan, L. L. & Zaher, H. S. Mol. Cell 68, 361–373 (2017). e5.

    Article  CAS  PubMed  Google Scholar 

  16. Ibrahim, F. et al. Nat. Struct. Mol. Biol. https://doi.org/10.1038/s41594-018-0042-8 (2018).

  17. Ingolia, N. T., Ghaemmaghami, S., Newman, J. R. S. & Weissman, J. S. Science 324, 218–223 (2009).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Guydosh, N. R. & Green, R. Cell 156, 950–962 (2014).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Lubas, M. et al. Cell Reps. 10, 178–192 (2015).

    Article  CAS  Google Scholar 

  20. Park, J. E., Yi, H., Kim, Y., Chang, H. & Kim, V. N. Mol. Cell 62, 462–471 (2016).

    Article  CAS  PubMed  Google Scholar 

  21. Bugaut, A. & Balasubramanian, S. Nucleic Acids Res. 40, 4727–4741 (2012).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Song, J., Perreault, J.-P., Topisirovic, I. & Richard, S. Translation 4, e1244031 (2016).

    Article  PubMed  PubMed Central  Google Scholar 

  23. Guydosh, N. R., Kimmig, P., Walter, P. & Green, R. eLife 6, e29216 (2017).

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

The authors thank M. Popp for comments on this manuscript. Work on mRNA decay in the Maquat laboratory is supported by R01 GM059614 and R37 GM074593, both to L.E.M.

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Authors and Affiliations

  1. Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, NY, USA

    Tatsuaki Kurosaki & Lynne E. Maquat

  2. Center for RNA Biology, University of Rochester, Rochester, NY, USA

    Tatsuaki Kurosaki & Lynne E. Maquat

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  1. Tatsuaki Kurosaki
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  2. Lynne E. Maquat
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Correspondence to Lynne E. Maquat.

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Kurosaki, T., Maquat, L.E. Molecular autopsy provides evidence for widespread ribosome-phased mRNA fragmentation. Nat Struct Mol Biol 25, 299–301 (2018). https://doi.org/10.1038/s41594-018-0048-2

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