Supplementary Figure 4: SNP-CLING signals of mouse loci. | Nature Structural & Molecular Biology

Supplementary Figure 4: SNP-CLING signals of mouse loci.

From: Spatiotemporal allele organization by allele-specific CRISPR live-cell imaging (SNP-CLING)

Supplementary Figure 4

(a) Gene-density (Gencode vM9) and chromosomal sizes (mm10) of the mouse genome. Loci on chromosome 1 (large chromosome), chromosome 7 and 11 (gene-rich chromosomes), chromosome 15 (gene-poor / small chromosome), and chromosome 18 (gene-poor / small chromosome), and Firre on chromosome X, were selected for SNP-CLING experiments. (b) Examples of allele-specific SNP-CLING signals (separate channels) of maternal and paternal loci in combination with rRNA staining of the nucleoli in female 129S1/CAST mEFs (n = 10, arrowheads = specific SNP-CLING foci of maternal and paternal alleles, or CLING foci for chr. 18 Puf-Pum1-iRFP670).

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