Supplementary Figure 1: Genotypization of SNP-CLING sgRNAs. | Nature Structural & Molecular Biology

Supplementary Figure 1: Genotypization of SNP-CLING sgRNAs.

From: Spatiotemporal allele organization by allele-specific CRISPR live-cell imaging (SNP-CLING)

Supplementary Figure 1

(a) Genome-wide distribution of suitable SNPs for SNP-CLING in human (mean distance = 181 bp, hg38), and in mouse (mean distance 332 bp, mm10, medians with 25th to 75th percentiles, 1.5x interquartile ranges, outliers = black dots). (b) Total numbers of suitable SNPs in human and mouse, and intergenic / intragenic distributions. (c) Heterozygous SNP substitutions in the second or third nucleotide of the PAM-sequences determined the usability of every sgRNA in SNP-CLING experiments on 129S1/CAST cells. For each allele, two sgRNAs specifically labelled every locus.

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