Supplementary Figure 5: CLING signals of mouse and human loci. | Nature Structural & Molecular Biology

Supplementary Figure 5: CLING signals of mouse and human loci.

From: Spatiotemporal allele organization by allele-specific CRISPR live-cell imaging (SNP-CLING)

Supplementary Figure 5

Examples of labeling various human and mouse loci with sgRNA pools. Four different sgRNAs were mixed in combinations of three to determine the best set for imaging (arrowheads = specific CLING foci). Before relevant experiments, the use of each sgRNA pool was specifically tested for (a) mouse loci in male mESCs (Firre on chromosome X was therefore detected as single voxel, n = 10), and (b) for coding genes in human in human RPE-1 or C28/I2 cells (n = 10).

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