Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Research Briefing
  • Published:

Widespread changes in alternative splicing in developing and adult mouse brain

In the first comprehensive mRNA isoform atlas of the developing and adult mouse brain, we discover that region and age influence the isoform repertoire of cell subtypes. We link peak cell type regulation to the critical development period and report attenuated levels in adulthood.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: Isoform variability in excitatory neurons.


  1. Aldridge, S. & Teichmann, S. A. Single cell transcriptomics comes of age. Nat. Commun. 11, 4307 (2020). A review of single-cell transcriptomics.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Raj, B. & Blencowe, B. J. Alternative splicing in the mammalian nervous system: recent insights into mechanisms and functional roles. Neuron 87, 14–27 (2015). A review summarizing known roles of alternative splicing in the brain.

    Article  CAS  PubMed  Google Scholar 

  3. Joglekar, A. et al. A spatially resolved brain region- and cell type-specific isoform atlas of the postnatal mouse brain. Nat. Commun. 12, 463 (2021). A study of isoform diversity in the postnatal mouse brain.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Gupta, I. et al. Single-cell isoform RNA sequencing characterizes isoforms in thousands of cerebellar cells. Nat. Biotechnol. 36, 1197–1202 (2018). An article outlining a method to sequence full-length RNA isoforms from single cells.

    Article  CAS  Google Scholar 

  5. Hardwick, S. A. et al. Single-nuclei isoform RNA sequencing unlocks barcoded exon connectivity in frozen brain tissue. Nat. Biotechnol. 40, 1082–1092 (2022). A method to sequence full-length isoforms from frozen nuclei and the insights it unlocks about the human brain.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Prjibelski, A. D. et al. Accurate isoform discovery with IsoQuant using long reads. Nat. Biotechnol. 41, 915–918 (2023). A computational tool to reconstruct RNA isoforms from bulk and single-cell long-read data.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Additional information

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

This is a summary of: Joglekar, A. et al. Single-cell long-read sequencing-based mapping reveals specialized splicing patterns in developing and adult mouse and human brain. Nat. Neurosci. (2024).

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Widespread changes in alternative splicing in developing and adult mouse brain. Nat Neurosci 27, 1040–1041 (2024).

Download citation

  • Published:

  • Issue Date:

  • DOI:


Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing