Extended Data Fig. 3: The POGlu neurons project onto S1HLGlu neurons. | Nature Neuroscience

Extended Data Fig. 3: The POGlu neurons project onto S1HLGlu neurons.

From: Distinct thalamocortical circuits underlie allodynia induced by tissue injury and by depression-like states

Extended Data Fig. 3

a, Schematic of PO injection of AAV-DIO-ChR2-mCherry in CaMKII-Cre mice. b, PO: representative image of mCherry labeling neurons by PO infusion of AAV-DIO-ChR2-mCherry. Scale bar, 200 µm. VP, IC, ZI, ACC: images representative of mCherry+ fibers in CaMKII-Cre mice with PO injection of AAV-DIO-ChR2-mCherry. Scale bars, 100 µm. c, Rabies-virus-mediated identification of monosynaptic inputs to POGlu neurons. d, Representative images showing input neurons in the contralateral S1HL, S2, IC, LH, and ZI. Scale bars, 100 µm. e, A series of coronal sections, ipsilateral to the site of injection, from a representative mouse showing the major monosynaptic inputs to S1HLGlu neurons. Scale bar, 1 mm. f, Normalized distributions of rabies-labeled input neurons across different brain areas in ipsilateral to the injection site. g, Schematic of the Cre-dependent retrograde trans-monosynaptic rabies virus tracing strategy in CaMKII-Cre mice. h, DsRed-labeled neurons expressions at different bregma sites in the thalamus of CaMKII-Cre mice. Scale bar, 200 µm. i, Schematic of the Cre-dependent retrograde trans-monosynaptic rabies virus tracing strategy in GAD2-Cre mice. j, DsRed-labeled neurons expressions at different bregma sites in the thalamus of GAD2-Cre mice. Scale bar, 200 µm. k, Left: data showing the number of DsRed-labeled neurons expressions at different bregma sites in the thalamus of CaMKII-Cre mice. Right: data showing the proportion of DsRed-labeled neurons co-localized with the glutamate or GABA immunofluorescence within the PO traced from the S1HL of CaMKII-Cre mice. l, Data showing the number of DsRed-labeled neurons expressions at different bregma sites in the thalamus of GAD2-Cre mice. VP, ventral posterior nucleus of thalamus; IC, insular cortex; ZI, zona incerta; ZIV, zona incerta, ventral part; ACC, anterior cingulate cortex; S2, secondary somatosensory cortex; LH, lateral hypothalamic area. All data are presented as the mean ± s.e.m.

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