Extended Data Fig. 9: Effects of manipulating POGlu→S1HLGlu and PFGlu→ACCGABA→Glu circuits on tissue injury- or chronic stress-associated allodynia, respectively. | Nature Neuroscience

Extended Data Fig. 9: Effects of manipulating POGlu→S1HLGlu and PFGlu→ACCGABA→Glu circuits on tissue injury- or chronic stress-associated allodynia, respectively.

From: Distinct thalamocortical circuits underlie allodynia induced by tissue injury and by depression-like states

Extended Data Fig. 9

a, Schematic of optogenetic experiments in CaMKII-Cre mice. b, c, Effects of optogenetic activation of PFGlu terminals in the ACC on behavioral tests in social interaction and tail-suspension upon CRS 3W mice (b) or CUS 10D mice (c) (b: n = 7 mice per group; CRS-mCherry: SI, t6 = 1.329, P = 0.232; TST, t6 = 1.132, P = 0.301; CRS-ChR2: SI, t6 = 0.473, P = 0.653; TST, t6 = 0.933, P = 0.387; c: CUS-mCherry, n = 7 mice; SI, t6 = 1.024, P = 0.345; TST, t6 = 0.454, P = 0.666; CUS-ChR2, n = 6 mice; SI, t5 = 0.413, P = 0.700; TST, t5 = 1.266, P = 0.261). d, Effects of optogenetic activation of PFGlu terminals in the ACC on pain threshold in CFA 3D (left) or SNI 7D (right) mice (n = 5 mice per group; CFA model: F1,8 = 0.357, P =0.567; SNI model: F1,8 = 0.732, P = 0.417). e, Schematic of chemogenetic experiments in CaMKII-Cre mice. f, Effects of chemogenetic activation of POGlu neurons on pain threshold in CRS 3W (left) or CUS 10D (right) mice (n = 5 mice per group; CRS model: F1,8 = 1.260, P =0.294; CUS model: F1,8 = 1.166, P = 0.312). g, Schematic of optogenetic experiments in CaMKII-Cre mice. h, Effects of optogenetic inhibition of POGlu terminals in the S1HL on pain threshold in CRS 3W (left) or CUS 10D (right) mice (n = 5 mice per group; CRS model: F1,8 = 0.533, P =0.486; CUS model: F1,8 = 0.053, P = 0.824). i, Schematic of optogenetic experiments in CaMKII-Cre mice. j, Effects of optogenetic inhibition of POGlu terminals in the ACC on pain threshold in CFA 3D (left) or SNI 7D (right) mice (n = 5 mice per group; CFA model: F1,8 = 0.122, P =0.736; SNI model: F1,8 = 0.087, P = 0.776). k, Schematic of optogenetics experiments in CaMKII-Cre mice. l, Effects of optogenetic activation of PFGlu terminals in the S1HL on pain threshold in CRS 3W (left) or CUS 10D (right) mice (n = 5 mice per group; CRS model: F1,8 = 5.03, P =0.055; CUS model: F1,8 = 0.099, P = 0.761). Significance was assessed by paired Student’s t-test in b and c, two-way repeated measures ANOVA with post hoc comparison between groups in d, f, h, j and l. All data are presented as the mean ± s.e.m. For detailed statistics information, see Supplementary Table 1.

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