Extended Data Fig. 8: Effects of manipulation of PF or ACC activity on chronic stress-associated allodynia. | Nature Neuroscience

Extended Data Fig. 8: Effects of manipulation of PF or ACC activity on chronic stress-associated allodynia.

From: Distinct thalamocortical circuits underlie allodynia induced by tissue injury and by depression-like states

Extended Data Fig. 8

a, Effects of chemogenetic activation of PFGlu neurons on behavioral tests in social interaction, tail suspension, forced swimming upon CRS 3W mice (mCherry, n = 10 mice; hM3Dq-mCherry, n = 5 mice; SI, t13 = 3.344, P = 0.005; TST, t13 = 2.724, P = 0.017; FST, t13 = 3.713, P = 0.003). b, Effects of chemogenetic activation of PFGlu neurons on behavioral tests in social interaction, tail-suspension, forced-swim upon CUS 10D mice. c, Schematic of chemogenetic experiments in CaMKII-Cre mice (mCherry, n = 8 mice; hM3Dq-mCherry, n = 6 mice; SI, t12 = 2.933, P = 0.013; TST, t12 = 3.812, P = 0.003; FST, t12 = 4.794, P < 0.001). d, Typical image showing the injection site within the ACC in CaMKII-Cre mice. Scale bar, 100 µm. e, Effects of chemogenetic inhibition of ACCGlu neurons on pain threshold in CRS 3W (left) or CUS 10D (right) mice (n = 5 mice per group; CRS model: F1,8 = 26.87, P < 0.001; CUS model: F1,8 = 50.96, P < 0.0001). f, Effects of chemogenetic inhibition of ACCGlu neurons on behavioral tests in social interaction, tail-suspension, forced-swim upon CRS 3W mice (n = 7 mice per group; SI, t12 = 3.333, P = 0.006; TST, t12 = 3.688, P = 0.003; FST, t12 = 5.126, P < 0.001). g, Effects of chemogenetic inhibition of ACCGlu neurons on behavioral tests in social interaction, tail-suspension, forced-swimming upon CUS 10D mice (mCherry, n = 10 mice; hM4Di-mCherry, n = 6 mice; SI, t14 = 6.959, P < 0.0001; TST, t14 = 3.990, P = 0.001; FST, t14 = 3.326, P = 0.005). h, Schematic of chemogenetic experiments in GAD2-Cre mice. i, Typical image showing the injection site within the ACC in GAD2-Cre mice. Scale bar, 100 µm. j, Effects of chemogenetic activation of ACCGABA neurons on pain threshold in CRS 3W (left) or CUS 10D (right) mice (n = 5 mice per group; CRS model: F1,8 = 20.30, P = 0.002; CUS model: F1,8 = 109.5, P < 0.0001). Significance was assessed by unpaired Student’s t-test in a, b, f and g, two-way repeated measures ANOVA with post hoc comparison between groups in e and j. All data are presented as the mean ± s.e.m. * P < 0.05, ** P < 0.01, *** P < 0.001. For detailed statistics information, see Supplementary Table 1.

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