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GENETIC TOOLS

CRISPR Rube Goldberg machines for visualizing cell lineage

A new technique developed by Garcia-Marques and colleagues uses CRISPR–Cas9 editing to activate an ordered sequence of fluorescent markers in stem cells and their progeny. These tools represent a new way to probe the spatial and temporal patterns of cell lineage progression.

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Fig. 1: CLADES enables sequential labeling of cell lineage.

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Correspondence to James A. Gagnon.

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Carey, C.M., Gagnon, J.A. CRISPR Rube Goldberg machines for visualizing cell lineage. Nat Neurosci 23, 1469–1471 (2020). https://doi.org/10.1038/s41593-020-0694-7

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