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CRISPR Rube Goldberg machines for visualizing cell lineage

A new technique developed by Garcia-Marques and colleagues uses CRISPR–Cas9 editing to activate an ordered sequence of fluorescent markers in stem cells and their progeny. These tools represent a new way to probe the spatial and temporal patterns of cell lineage progression.

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Fig. 1: CLADES enables sequential labeling of cell lineage.


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Correspondence to James A. Gagnon.

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Carey, C.M., Gagnon, J.A. CRISPR Rube Goldberg machines for visualizing cell lineage. Nat Neurosci 23, 1469–1471 (2020).

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