Extended Data Fig. 1: Optogenetically identified and putative cholinergic neurons behave similarly. | Nature Neuroscience

Extended Data Fig. 1: Optogenetically identified and putative cholinergic neurons behave similarly.

From: Distinct synchronization, cortical coupling and behavioral function of two basal forebrain cholinergic neuron types

Extended Data Fig. 1

a, Average auto-correlogram of Burst-BFCN-SBs (red), Burst-BFCN-PLs (orange) and Reg-BFCNs (green). Left, optogenetically identified; right, putative. While nominal normalized magnitudes may differ due to varying noise levels and moderate sample sizes, the auto-correlation curves are qualitatively similar. Solid lines, mean; shading, s.e.m. b, Response to punishment of identified cholinergic neurons (left, identified NB; right, identified HDB). Solid lines, mean; shading, s.e.m. c, Response to punishment of putative cholinergic neurons. HDB neurons showed somewhat slower and more variable responses. Note also the longer response latencies of two regular pChAT neurons. Solid lines, mean; shading, s.e.m. d, Burst index vs. relative refractory period for identified (circle; red, n = 26 Burst-BFCN-SBs; orange, n = 17 Burst-BFCN-PLs; green, n = 13 Reg-BFCNs) and putative (triangle; red, n = 12 Burst-BFCN-SBs; orange, n = 8 Burst-BFCN-PLs; green, n = 2 Reg-BFCNs) cholinergic neurons. e, Pearson’s correlation between theta index and relative refractory period. No systematic difference between identified (circle; red, n = 26 Burst-BFCN-SBs; orange, n = 17 Burst-BFCN-PLs; green, n = 13 Reg-BFCNs) and putative (triangle; red, n = 12 Burst-BFCN-SBs; orange, n = 8 Burst-BFCN-PLs; green, n = 2 Reg- BFCNs) cholinergic neurons were detected (p = 0.0007 for n = 15 Reg-BFCNs, one-sided F-test, F(1,13) = 19.67). f, Baseline firing rate did not show systematic differences between identified (circle; red, n = 26 Burst-BFCN-SBs; orange, n = 17 Burst-BFCN-PLs; green, n = 13 Reg-BFCNs) and putative (triangle; red, n = 12 Burst-BFCN-SBs; orange, n = 8 Burst-BFCN-PLs; green, n = 2 Reg-BFCNs) cholinergic neurons.

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