Extended Data Fig. 5: Discovery of a fundamental transcriptional bifurcation among γ motor neurons. | Nature Neuroscience

Extended Data Fig. 5: Discovery of a fundamental transcriptional bifurcation among γ motor neurons.

From: Single-cell transcriptomic analysis of the adult mouse spinal cord reveals molecular diversity of autonomic and skeletal motor neurons

Extended Data Fig. 5

a, UMAP with 3 subclustered γ motor neurons populations. b, Novel marker gene expression across γ motor neuron subpopulations. Dot size is proportional to the percent of each cluster expressing the marker gene, while blue color intensity is correlated with expression level. c, Average log-normalized expression of genes enriched in γ* motor neurons over γ overlaid on UMAP. α, γ, and γ* populations are labeled. d, Average log-normalized expression of genes enriched in γ motor neurons over γ* overlaid on UMAP. α, γ, and γ* populations are labeled. e-h, Average expression of novel γ markers Stxbp6 (e) and Plch1 (f), as well as novel γ* markers Pard3b (g) and Creb5 (h) by cluster. i, Representative in situ hybridization against Htr1d/Creb5/Stxbp6 in transverse spinal cord shows mutual exclusion of novel markers Creb5 and Stxbp6 in Htr1d + cells. n = 4 biologically independent animals. Scale bar=20 µm (inset) and 200 µm (overview). j, Representative in situ hybridization against Htr1d/Pard3b/Stxbp6 in transverse spinal cord shows mutual exclusion of novel markers Pard3b and Stxbp6 in Htr1d + cells. Arrowheads label canonical γ motor neurons and *labels γ*. n = 5 biologically independent animals. Scale bar=20 µm (inset) and 100 µm (overview). Differentially expressed genes determined by Wilcoxon rank sum test implementation in Seurat and adjusted for multiple comparisons (Bonferroni method) (p_adj<0.01, log2-fold change >0.5).

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