Extended Data Fig. 5: Photoinhibition of SOM+ interneurons abolishes affective state discrimination. | Nature Neuroscience

Extended Data Fig. 5: Photoinhibition of SOM+ interneurons abolishes affective state discrimination.

From: Somatostatin interneurons in the prefrontal cortex control affective state discrimination in mice

Extended Data Fig. 5

Related to Fig. 4. a, Top, representative images of viral expression in the mPFC (in rostro-caudal order) after injection with AAV-EF1a-DIO-eNpHR3.0-eYFP. Bottom, reconstruction of viral expression and location of optical fibers. Red areas represent the expression (higher expression = darker color) of AAV-EF1a-DIO-eNpHR3.0-eYFP in SOM-cre mice. Findings were replicated in four independent experiments with similar results. b, Increased exploration toward the odor of the relieved demonstrators compared to the neutral in light off and light on conditions (two-way RM ANOVA, affective state (relief, neutral): F(1,14)=12.65, p<0.005, n=8 mice), and no effects of SOM+ photo-inhibition (F(1,14)=0, p=0.99). c, Avoidance of the odor of the stressed demonstrators in in light off and light on conditions (two-way RM ANOVA, affective state (stress, neutral): F(1,10)=76.21, p<0.0001, n=6 mice), which SOM+ photo-inhibition did not change (F(1,10)=0.33, p=0.576). d and e, SOM+ photoinhibition with continuous green light for two minutes did not induce any gross motor change in both relief (two-tailed multiple t-test, Bonferroni correction, distance travelled: t=0.57, df=12, p=0.576; average speed: t=0.50, df=12, p=0.625) and stress conditions (distance travelled: t=0.69, df=12, p=0.498; average speed: t=0.45, df=12, p=0.658). Data derived from n=7 mice in each condition (relief, stress). f, Exploration of the relieved demonstrator was paired to SOM+ photo-inhibition throughout the test (n=8 mice). g, No preference to spend more time with the relieved demonstrator during photo-inhibition of SOM+, on the first two minutes of ADT (two-tailed multiple t-test, Bonferroni correction: t=0.47, df=14, p>0.999). h, No change of number of visits to each demonstrator during photoinhibition of SOM+ (two-tailed multiple t-test, Bonferroni correction: t=0.88, df=14, p>0.999). i, SOM+ photoinhibition paired to exploration of the relieved demonstrators did not induce any gross motor changes. j, Exploration of the relieved demonstrator was paired to SOM+ photo-inhibition throughout the test (n=9 mice). k, No difference in time spent with the two demonstrators during photoinhibition of SOM+ (two-tailed multiple t-test, Bonferroni correction: t=0.19, df=16, p>0.999). l, No difference of number of visits to stressed and neutral demonstrator during inhibition of SOM+ (two-tailed multiple t-test, Bonferroni correction: t=0.11, df=16, p>0.999). m, SOM+ photoinhibition paired to exploration of the stressed demonstrators did not induce any gross motor changes. n, Exploration of one naïve “neutral” demonstrator (“neutral 1”) was paired to SOM+ photoinhibition throughout the ADT (counterbalanced, left or right, across observers, continuous green light). Data derived from n=8 mice. SOM+ photoinhibition did not induce social discrimination or avoidance conditions (two-way RM ANOVA, time x light (on,off): F(359,2513)=0.21, p>0.999). No discrimination of the two neutral demonstrators without light stimulation (“No light”, two-way RM ANOVA, time x light (on,off): F(359,2513)=0.19, p>0.999). Bar and line graphs show mean ± s.e.m. * p<0.05.

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