Extended Data Fig. 4: Photoinhibition of PV+ interneurons does not affect affective state discrimination. | Nature Neuroscience

Extended Data Fig. 4: Photoinhibition of PV+ interneurons does not affect affective state discrimination.

From: Somatostatin interneurons in the prefrontal cortex control affective state discrimination in mice

Extended Data Fig. 4

Related to Fig. 3. a, Top, representative images of viral expression in the mPFC after injection with AAV-EF1a-DIO-eNpHR3.0-eYFP. Bottom, reconstruction of viral expression and location of optical fibers. Red areas represent the expression (higher expression = darker color) of AAV-EF1a-DIO-eNpHR3.0-eYFP in PV-cre mice. Findings were replicated in two independent experiments with similar results. Data derived from n=7 mice. b, PV-cre mice were tested in the ADT with one relieved and one neutral demonstrator. Photo-inhibition was performed for 2 minutes, from the beginning of the test, using continuous green light. c, No effect of PV+ photoinhibition on latency to made the first visit to the relieved demonstrator (two-way RM ANOVA, affective state (relief, neutral): F(1,12)=7.18, p=0.020). d, Optical inhibition of PV+ did not modify the number of visits to each demonstrator (two-way RM ANOVA, affective state (relief, neutral) x light (off,on): F(1,24)=0.38, p=0.541). e, PV-cre mice were tested in the ADT with one stressed and one neutral demonstrator. Photoinhibition was performed for 2 minutes, from the beginning of the test, using continuous green light. Data derived from n=7 mice. f, No effect of PV+ photoinhibition on latency to made the first visit to the stressed demonstrator (two-way RM ANOVA, affective state (stress, neutral): F(1,12)=12.75, p=0.003). g, Optical inhibition of PV+ did not modify the number of visits to each demonstrator (two-way RM ANOVA, affective state (stress, neutral) x light (off,on): F(1,24)=0.30, p=0.587). h and i, Optical inhibition of PV+ did not induce gross motor deficits during ADT in both relief (two-tailed multiple t-test, Bonferroni correction, distance travelled: t=0.35, df=12, p=0.730; average speed: t=1.10, df=12, p=0.290) and stress conditions (distance travelled: t=1.19, df=12, p=0.254; average speed: t=0.47, df=12, p=0.640). Bar and line graphs show mean ± s.e.m. * p<0.05.

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