Supplementary Figure 2: Divergent binding of Ascl1 and Neurog2 is not an artifact. | Nature Neuroscience

Supplementary Figure 2: Divergent binding of Ascl1 and Neurog2 is not an artifact.

From: Proneural factors Ascl1 and Neurog2 contribute to neuronal subtype identities by establishing distinct chromatin landscapes

Supplementary Figure 2

ChIP-seq heatmap with all sites identified in 12 h Ascl1 and Neurog2 datasets shows the divergent binding pattern when 10 k (Fig. 2) or all sites are analyzed (n = 3). b, Ascl1 and Neurog2 bind to largely non-overlapping sites even at 48 h after induction. ChIP-seq heatmap with top 10k sites identified in 48 h Ascl1 and Neurog2 datasets shows that the late binding of Ascl1 and Neurog2 is also divergent (n = 2). c, ChIP-seq heatmap with all sites identified in 48 h Ascl1 and Neurog2 datasets showing that the late divergent binding is still retained when all sites are analyzed (n = 2). d, e, Comparison of Ascl1 ChIP-seq experiments across published datasets in mouse embryonic fibroblasts (MEFs) (d), and ESCs (e). Heatmaps display shared binding sites (that is peaks called in both experiments) and significantly differentially bound sites across experiments. f, ChIP-seq and RNA-seq genome browser snapshots displaying gene expression and differential binding of Ascl1 and Neurog2 at the Dll1 locus. g, Comparison of Ascl1 and Neurog2 binding across 12 h and 48 h time-points. Table displays counts of shared binding sites (that is peaks called in both experiments) and significantly differentially bound sites across experiments.

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