Supplementary Figure 6: Examples of shared targets that have differential Ascl1 or Neurog2 binding. | Nature Neuroscience

Supplementary Figure 6: Examples of shared targets that have differential Ascl1 or Neurog2 binding.

From: Proneural factors Ascl1 and Neurog2 contribute to neuronal subtype identities by establishing distinct chromatin landscapes

Supplementary Figure 6

a, Comparison of endogenous Brn2 ChIP-seq in mESC (this paper) with exogenous Brn2 in MEFs programming when expressed exogenously with Ascl1 and Myt1l (iBAM – Wapinski et al25). Heatmaps display shared binding sites (that is peaks called in both experiments) and significantly differentially bound sites across experiments. b, Maximum motif log-likelihood scores of downstream TFs shows weaker enrichment of their respective motifs in iA > iN (left) or iN > iA (right) sites that have E-box motifs as opposed to their top 10 K binding sites (150 bp window around sites) (n = 2 independent cell differentiations) c, tSNE plots showing the cells that express shared targets in iA (top cluster) and iN (bottom cluster) neuron clusters (top panel / n = 1 cell differentiation) that have differential Ascl1 or Neurog2 binding (genome browser shots – bottom panel / n = 3 independent cell differentiations).

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