Supplementary Figure 5: Global chromatin accessibility dynamics mimic the divergent-to-convergent gene expression dynamics in Ascl1- and Neurog2-induced neurogenesis. | Nature Neuroscience

Supplementary Figure 5: Global chromatin accessibility dynamics mimic the divergent-to-convergent gene expression dynamics in Ascl1- and Neurog2-induced neurogenesis.

From: Proneural factors Ascl1 and Neurog2 contribute to neuronal subtype identities by establishing distinct chromatin landscapes

Supplementary Figure 5

a, Global comparison of sites that differentially gained or lost accessibility at 12 h (top) and 48 h (bottom) iA and iN neurons with respect to EB 0 h. Note the increased shared changes by 48 h (p-val cut-off < 0.05) (n = 2 independent cell differentiations). b, Comparison of differentially accessible regions in iAscl1 EBs (this paper) to iAscl1 in MEFs (Wapinski et al45). c, Time-series ATAC-seq heatmaps showing the dynamics of the accessibility gain at the differentially bound and shared sites of Ascl1 and Neurog2 along with the accessibility dynamics in the iA[N]bHLH chimeric TF line. Note that the A[N]bHLH chimera has increased ATAC-seq read counts at the Neurog2-preferred sites (N > A) both at 12 h and 48 h.

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