Supplementary Figure 12: OPC perivascular clusters resolve over time. | Nature Neuroscience

Supplementary Figure 12: OPC perivascular clusters resolve over time.

From: Aberrant oligodendroglial–vascular interactions disrupt the blood–brain barrier, triggering CNS inflammation

Supplementary Figure 12

OPCs perivascular clusters resolve over time with reinvestment of astrocyte endfeet on blood vessels and reduction in microglial activation. (a) Use of Olig2-cre:APCfl/fl:TdTomato mice, to label OPC perivascular clusters in red (arrows), shows a decrease in cluster size around CD31+ vessels in corpus callosum through postnatal times P9, P12, P16 and P30. (b) Use of Olig2-cre:APCfl/fl:Aldh1l1-GFP at P9, P12, P16 and P30 in the corpus callosum shows a reduction in perivascular clusters over time (labelled with dapi, arrows) and reinvestment of GFP-labelled astrocyte endfeet on CD31+ vasculature. (c) Quantification of OPC cluster frequencies during development in Olig2-cre:APCfl/fl mouse CC at P9, P12, P16, P20 and P30 (n=6 animals at each time point; all statistical analyses compared to P9, P12 vs. P9 ns p = 0.1948, P16 vs. P9 * p = 0.141, P20 vs. P9 **** p = 2.25 E-5, P30 vs. P9 **** p = 2.19 E-6). Data were analyzed by unpaired two-sided Student’s t test. (d) Use of Olig2-cre:APCfl/fl corpus callosum at P9, P12, P16 and P30 shows a reduction in activated microglia (labelled with F4/80) over time around perivascular clusters (labelled with dapi). Scale bars, 80 μm (a, d), 30 μm (b). * P < 0.05, **** P < 0.0001. Values are mean ± s.d. For all staining results, experiments were repeated at least three times independently with similar results.

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