(a) Pdgfrβ staining (marking pericytes) outlines a vessel (in longitudinal section) in P9 corpus callosum of Olig2-cre:APCfl/fl:TdTomato:Aldh1l1-GFP, and pericyte vascular coverage appears unaffected (arrow in a) by the presence of an OPC perivascular cluster (TdTomato+). (b) Whilst astrocyte endfeet (green, filled arrow) are displaced from vessels with OPC perivascular clusters (red, TdTomato+) in P9 CC of Olig2-cre:APCfl/fl:TdTomato:Aldh1l1-GFP, pericytes labelled with Pdgfrβ (white) seem unaltered, and outline a blood vessel (unfilled arrow). (c) Examples of transverse sections through vessels in P9 corpus callosum of Olig2-cre:APCfl/fl:TdTomato showing intact Pdgfrβ+ staining marking pericytes in the presence of OPC perivascular clusters (TdTomato+). (d) There is no significant difference in the Pdgfrβ+ pericyte coverage of CD31+ vessels in P9 corpus callosum of Olig2cre:APCfl/fl versus control APCfl/fl mice (n=5 animals; APC fl/fl vs. Olig2 cre:APC fl/fl, ns p = 0.7158). Data were analyzed by unpaired two-sided Student’s t test. Scale bars, 20 μm in all panels. Values are mean ± s.d. For all staining results, experiments were repeated at least three times independently with similar results.