(a-b) OPC clusters have excessive Wnt tone evidenced by significantly increased Axin2 mRNA expression (arrows in b) in PDGFRα-creERT2:APC fl/fl at 10dpl lesion edges, which is not seen in APCfl/fl controls (a). (c-d) Axin2 expressing OPC clusters (arrows) accumulate on vasculature (CD31+) at 10dpl lesion edges in PDGFRα-creERT2:APC fl/fl (c), and co-localize with expression of Olig2 protein (d, arrow). (e-f) OPC clusters (labelled with PDGFRα) are labelled with GFP expression and can be seen around vasculature (CD31+) at 10dpl lesion edges in PDGFRα-creERT2:APCfl/fl:Rosa-GFP (arrows in f), but are not seen in PDGFRα-creERT2:APCfl/+:Rosa-GFP (e). Scale bars, 20 μm (a, d, e). For all staining results, experiments were repeated at least three times independently with similar results.