Supplementary Figure 4: Characterization of labeled cells in lesioned NG2creERT: tdTomato mice. | Nature Neuroscience

Supplementary Figure 4: Characterization of labeled cells in lesioned NG2creERT: tdTomato mice.

From: Aberrant oligodendroglial–vascular interactions disrupt the blood–brain barrier, triggering CNS inflammation

Supplementary Figure 4

(a, b) Acute slice cultures of 1.5 day lesioned adult NG2creERT: TdTomato mice spinal cord dorsal funiculus were used for live imaging. PDGFRα (enlarged in b) staining shows that TdTomato labelled cells following tamoxifen treatment were predominantly OPCs at lesion edges. Blood vessels were labeled by tail vein injection of the Fluorescent-lectin dye. Arrows show close cell body association of OPCs with vasculature at lesion edges in these mice. (c) Quantification of TdTomato labelled cells in acute slice cultures of 1.5 day lesioned adult NG2creERT: TdTomato after tamoxifen treatment. The majority of cells were OPCs (n=6 animals, 86.13±9.17 % were Olig2 positive, 80.21±10.70 % cells were PDGFRα positive) with a minority of pericytes labelled (9.04±4.65% cells were PDGFRβ positive). (d) Recombination efficiency following tamoxifen treatment of NG2creERT:TdTomato in pericytes was significantly (n=6 animals, **** P =1.39 E-7) lower (10.90±1.75 %) compared to recombination in OPCs (71.19±4.14 %). Data were analyzed by unpaired two-sided Student’s t test. Scale bars, 30 μm (a, b). **** P < 0.0001. Values are mean ± s.d. For all staining results, experiments were repeated at least three times independently with similar results.

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