Supplementary Figure 2: Insufficient staining using previously reported constructs. | Nature Neuroscience

Supplementary Figure 2: Insufficient staining using previously reported constructs.

From: Multiplexed peroxidase-based electron microscopy labeling enables simultaneous visualization of multiple cell types

Supplementary Figure 2

(a) LM image of HEK293T cells after transfection with HRP-TM. n = 3 experiments. (b) EM image showing plasma membrane labeling in a HEK293T cell transfected with HRP-TM (asterisk). n = 2 experiments. (c) LM image of the cortex after parenchymal injection of AAV1-HRP-TM. Staining could be observed with LM. n = 4 animals and experiments. (d) EM image of the cortex after parenchymal injection of AAV1-HRP-TM. Little if any discernible DAB staining was observed. n = 2 animals and experiments. Scale bars: a: 100 μm, b: 2 μm, c: 500 μm, d: 0.5 μm.

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