Supplementary Figure 4: Excessively high concentrations of DAB could cause staining artifacts. | Nature Neuroscience

Supplementary Figure 4: Excessively high concentrations of DAB could cause staining artifacts.

From: Multiplexed peroxidase-based electron microscopy labeling enables simultaneous visualization of multiple cell types

Supplementary Figure 4

(a, b) EM images of the spinal cord dorsal horn of the same animal after systemic transduction of AAV9-Matrix-dAPEX2 stained with 0.003% hydrogen peroxide and 1 mg/mL DAB (a) or 0.3 mg/mL DAB (b). The ultrastructure of the IMS was better preserved with 0.3 mg/mL DAB staining. n = 2 animals and experiments. Scale bar: 0.2 μm.