Supplementary Figure 5: Resemblances of the developmental gene-expression profile between the organoids and the fetal brain. | Nature Neuroscience

Supplementary Figure 5: Resemblances of the developmental gene-expression profile between the organoids and the fetal brain.

From: Cerebral organoids at the air–liquid interface generate diverse nerve tracts with functional output

Supplementary Figure 5

a. Heatmaps show normalized layer-specific gene expression in ALI-COs and b. in 12–13 week old human fetal brain cells24 ordered by pseudotime. Colour bars reflect gene expression levels. c. Matrices demonstrate Pearson correlation of gene expression for developmental layer-specific markers in the ALI-COs in comparison with d. and e. datasets5,24 derived from two other studies on 3D cerebral organoids (blue = negative correlation, yellow = positive correlation) comprising 508 cells from 9 brain organoid samples (Camp et al. study) and 66,889 cells from 19 brain organoids (Quadrato et al. study). Colour bar represents correlation values. f. Feature plots for the top 5 differentially expressed genes per cluster in the organoids. g. Featuremap shows cell group specific areas in different colours and feature plots demonstrate the distribution of cells expressing region-specific genes across multiple clusters.

Back to article page